The Prophylactic Activity of Punica granatum L. mesocarp Protects Preadipocytes against Ribosylated BSA-Induced Toxicity
Version: 1,
Uploaded by: MRIC Publisher 02,
Date Uploaded:
26 October 2021
Warning
You are about to be redirected to a website not operated by the Mauritius Research and Innovation Council. Kindly note that we are not responsible for the availability or content of the linked site. Are you sure you want to leave this page?
Objective: It was aimed at comparing the glycating capacities of glucose and ribose in bovine
serum albumin (BSA) and anti-glycation activity of pomegranate mesocarp extract (PME). The protective mechanism of PME against ribosylated BSA (BSARIB)-induced toxicity was also investigated.
Methods: BSA was incubated with glucose or ribose in the presence or absence of PME for
15 days. In preadipocytes pretreated with PME, cell viability, ROS production, lipid peroxidation
and mitochondrial membrane potential were investigated following 1, 6, 12, 18 and 24 h exposure
to BSARIB. Nuclear translocation of NFjB was assessed at 1 h and 24 h of BSARIB insult.
Accumulation of oxidized proteins, activities of intrinsic antioxidant enzymes and IL-6 secretion
were also determined after 24 h exposure to BSARIB.
Results: Ribose was a harsher glycating agent as compared to glucose and PME showed strong
anti-glycation activity by suppressing (P< 0.05) the increase in levels of fluorescent AGEs, Amadori
products, protein carbonyl and advanced oxidation protein products (AOPP). In preadipocytes,
BSARIB potentiated pro-apoptotic activity by inhibiting the nuclear translocation of NFjB. BSARIB
induced a time dependent decrease in cell viability, which was significantly suppressed (P< 0.05)
by PME. The extract also significantly reduced (P< 0.05) the time dependent increase in ROS level
and associated lipid peroxidation as well as loss in mitochondrial membrane potential caused by
BSARIB. PME also counteracted the BSARIB-induced accumulation of oxidized proteins, decrease in
intrinsic antioxidant activity and IL-6 over-secretion.
Conclusions: PME showed anti-glycation activity and afforded protection against BSARIB-induced
toxicity, oxidative stress and inflammation in preadipocytes.